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KMID : 0665220160290060923
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Korean Journal of Food and Nutrition
2016 Volume.29 No. 6 p.923 ~ p.929
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Protective Effect of PineXol® on Hydrogen Peroxide-induced Apoptosis on SK-N-MC Cells and Focal Ischemia Rodent Models
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Hong Soon-O
Han Kyung-Hoon
Lee Seung-Hee
Kim Doh-Hee
Song Kwan-Young
Han Sung-Hee
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Abstract
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The purpose of this study was to evaluate the protective effect of PineXol® on H2O2-induced cell death in SK-N-MC cells, and in early stage focal ischemia rodent model. SK-N-MC cells were pre-treated with 200 μM H2O2 or various concentrations of PineXol® (10, 30, and 50 pg/mL) for 24 h, and then exposed to H2O2 for 3 h. Cell death was assessed by the CCK-8 assay, reactive oxygen species (ROS) assay, and lactate and dehydrogenase (LDH) release assay. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) expressions were also analyzed by western blotting. Focal ischemia rodent model was used as the in vivo model, and different concentrations of PineXol® (1, 10, and 100 mg/kg) were administered. One week after administration, reduction of infarct volume was analyzed by TTC staining. Cell viability of H2O2-treated SK-N-MC cells significantly increased by pre-treatment of PineXol® (p<0.05). PineXol® pre-treatment also induced significant decrease of ROS and LDH expressions. However, PineXol® did not affect the infarct volume. These results suggest that PineXol® has significant neuroprotective effect in vitro, but statistical significance was not confirmed in the in vivo focal ischemia model.
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KEYWORD
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PineXol®, SK-N-MC cell, focal ischemia rodent models, neuroprotection
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